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Double Regulation of Supplement-Detoxification on Diabetic Atherosclerosis by Inhibiting Excessive Autophagy

Received: 9 January 2023     Accepted: 6 February 2023     Published: 9 February 2023
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Abstract

Objecive To explore the mechanism of Huoxue Jiedu Jiangtang recipe (HJJR) in regulating the hyperactivation of endoplasmic reticulum stress (ERS) autophagy PERK-eIF2a-LC3 pathway, and reveal its prevention for diabetic atherosclerosis. Methods First, the rats were injected with streptozotocin intraperitoneally to make the diabetic models, and then the aortic balloon injury combined with high-fat feeding were used to make the models of atherosclerosis who were randomly divided into model group, western medicine group (Gliquidone + Benazepril), low-dose HJJR group (HJJR1), high-dose HJJR group (HJJR2), and accepted corresponding drugs for 8 weeks respectively. Another blank group was used as control. The changes of serum glycosylated hemoglobin (GHb), homocysteine (Hcy) and angiotensinII (Ang1I) were compared. HE staining was used to detect aortic pathology. The mRNA transcription of PRK-like endoplasmic reticulum kinase (PERK), eucaryotic translation initation factor 2α (eIF2α) and CCAAT / Enhance-Binding Protein Homologous Protein (CHOP) were tested by reverse- transcription polymerase chain reaction (RT-PCR). Western blot were used to detect the expression of autophagy activating protein ATG6 (Beclin-1), autophagy protein microtubule-associated protein light chain LC3-1, LC3-II and LC3-II/LC3-I. Results Compared with the model group, after the drug intervention, the aortic tissue injury in each drug group were reduced, and the improvement in the Chinese medicine group was significantly better than that of the western medicine group (P < 0.05). All treatment groups could significantly lowed the levels of serum GHb, Hcy, and Ang1I (P < 0.05), could down regulate the transcription level of PERK, eIF2α and CHOP mRNA (P < 0.05); and decreased the amount of Beclin 1, LC3-II and LC3-II/LC3-1 autophagy protein in aortic cells (P < 0.05). The effect of HJJF group was better, and it was more significant with the increase of HJJF dose. Conclusion HJJR can protect arteries by inhibiting ERS caused by metabolic disorder in diabetes, correcting the homeostasis affecting ER, reducing the overreaction of PERK-eIF2-LC3, and alleviating autophagy effect.

Published in World Journal of Public Health (Volume 8, Issue 1)
DOI 10.11648/j.wjph.20230801.13
Page(s) 15-22
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Diabetes Mellitus, Atherosclerosis, Huoxue Jiedu Jiangtang Recipe, Endoplasmic Reticulum Stress, Autophagy

References
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[3] Fu Xianzhao, Huang Zhenfeng, Huang Wenhua, et al. Review and prospect of using traditional Chinese medicine drugs”supplementing Qi and nourishing Yin, activating blood circulation and detoxifying” for prevention and treatment of diabetes mellitus complicated with acutecoronary syndrome. ChinJ TCM WM Crit Care, 2017, 24 (5): 547-551.
[4] Fu Xianzhao, Xu Jing, Li Chunyan, et al. Efects of Huoxue Jiedu Jiang tang formulation on the balance between pro-and anti-inf lammatory cytokines in non-revascularization patients with diabetic acute coronary syndrome [J]. ChinJ TCM WM Crit Care, 2016, 23 (1): 6-10.
[5] ZHENG Wanqiu, LI Shuo, WU Jinfeng, et al. Mechanism of endoplasmic reticulum autophagy [J]. J Clin Pathol Res, 2022, 42 (6): 1460-2485.
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  • APA Style

    Fu Xianzhao, Jiang Xiaofeng, Chen Jiajun, Shen Yaya. (2023). Double Regulation of Supplement-Detoxification on Diabetic Atherosclerosis by Inhibiting Excessive Autophagy. World Journal of Public Health, 8(1), 15-22. https://doi.org/10.11648/j.wjph.20230801.13

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    ACS Style

    Fu Xianzhao; Jiang Xiaofeng; Chen Jiajun; Shen Yaya. Double Regulation of Supplement-Detoxification on Diabetic Atherosclerosis by Inhibiting Excessive Autophagy. World J. Public Health 2023, 8(1), 15-22. doi: 10.11648/j.wjph.20230801.13

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    AMA Style

    Fu Xianzhao, Jiang Xiaofeng, Chen Jiajun, Shen Yaya. Double Regulation of Supplement-Detoxification on Diabetic Atherosclerosis by Inhibiting Excessive Autophagy. World J Public Health. 2023;8(1):15-22. doi: 10.11648/j.wjph.20230801.13

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  • @article{10.11648/j.wjph.20230801.13,
      author = {Fu Xianzhao and Jiang Xiaofeng and Chen Jiajun and Shen Yaya},
      title = {Double Regulation of Supplement-Detoxification on Diabetic Atherosclerosis by Inhibiting Excessive Autophagy},
      journal = {World Journal of Public Health},
      volume = {8},
      number = {1},
      pages = {15-22},
      doi = {10.11648/j.wjph.20230801.13},
      url = {https://doi.org/10.11648/j.wjph.20230801.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjph.20230801.13},
      abstract = {Objecive To explore the mechanism of Huoxue Jiedu Jiangtang recipe (HJJR) in regulating the hyperactivation of endoplasmic reticulum stress (ERS) autophagy PERK-eIF2a-LC3 pathway, and reveal its prevention for diabetic atherosclerosis. Methods First, the rats were injected with streptozotocin intraperitoneally to make the diabetic models, and then the aortic balloon injury combined with high-fat feeding were used to make the models of atherosclerosis who were randomly divided into model group, western medicine group (Gliquidone + Benazepril), low-dose HJJR group (HJJR1), high-dose HJJR group (HJJR2), and accepted corresponding drugs for 8 weeks respectively. Another blank group was used as control. The changes of serum glycosylated hemoglobin (GHb), homocysteine (Hcy) and angiotensinII (Ang1I) were compared. HE staining was used to detect aortic pathology. The mRNA transcription of PRK-like endoplasmic reticulum kinase (PERK), eucaryotic translation initation factor 2α (eIF2α) and CCAAT / Enhance-Binding Protein Homologous Protein (CHOP) were tested by reverse- transcription polymerase chain reaction (RT-PCR). Western blot were used to detect the expression of autophagy activating protein ATG6 (Beclin-1), autophagy protein microtubule-associated protein light chain LC3-1, LC3-II and LC3-II/LC3-I. Results Compared with the model group, after the drug intervention, the aortic tissue injury in each drug group were reduced, and the improvement in the Chinese medicine group was significantly better than that of the western medicine group (P Conclusion HJJR can protect arteries by inhibiting ERS caused by metabolic disorder in diabetes, correcting the homeostasis affecting ER, reducing the overreaction of PERK-eIF2-LC3, and alleviating autophagy effect.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Double Regulation of Supplement-Detoxification on Diabetic Atherosclerosis by Inhibiting Excessive Autophagy
    AU  - Fu Xianzhao
    AU  - Jiang Xiaofeng
    AU  - Chen Jiajun
    AU  - Shen Yaya
    Y1  - 2023/02/09
    PY  - 2023
    N1  - https://doi.org/10.11648/j.wjph.20230801.13
    DO  - 10.11648/j.wjph.20230801.13
    T2  - World Journal of Public Health
    JF  - World Journal of Public Health
    JO  - World Journal of Public Health
    SP  - 15
    EP  - 22
    PB  - Science Publishing Group
    SN  - 2637-6059
    UR  - https://doi.org/10.11648/j.wjph.20230801.13
    AB  - Objecive To explore the mechanism of Huoxue Jiedu Jiangtang recipe (HJJR) in regulating the hyperactivation of endoplasmic reticulum stress (ERS) autophagy PERK-eIF2a-LC3 pathway, and reveal its prevention for diabetic atherosclerosis. Methods First, the rats were injected with streptozotocin intraperitoneally to make the diabetic models, and then the aortic balloon injury combined with high-fat feeding were used to make the models of atherosclerosis who were randomly divided into model group, western medicine group (Gliquidone + Benazepril), low-dose HJJR group (HJJR1), high-dose HJJR group (HJJR2), and accepted corresponding drugs for 8 weeks respectively. Another blank group was used as control. The changes of serum glycosylated hemoglobin (GHb), homocysteine (Hcy) and angiotensinII (Ang1I) were compared. HE staining was used to detect aortic pathology. The mRNA transcription of PRK-like endoplasmic reticulum kinase (PERK), eucaryotic translation initation factor 2α (eIF2α) and CCAAT / Enhance-Binding Protein Homologous Protein (CHOP) were tested by reverse- transcription polymerase chain reaction (RT-PCR). Western blot were used to detect the expression of autophagy activating protein ATG6 (Beclin-1), autophagy protein microtubule-associated protein light chain LC3-1, LC3-II and LC3-II/LC3-I. Results Compared with the model group, after the drug intervention, the aortic tissue injury in each drug group were reduced, and the improvement in the Chinese medicine group was significantly better than that of the western medicine group (P Conclusion HJJR can protect arteries by inhibiting ERS caused by metabolic disorder in diabetes, correcting the homeostasis affecting ER, reducing the overreaction of PERK-eIF2-LC3, and alleviating autophagy effect.
    VL  - 8
    IS  - 1
    ER  - 

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Author Information
  • The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China

  • The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China

  • The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China

  • The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China

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